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After washing, sections were incubated with fluorescent secondary antibodies for 1 h at room temperature, washed again, mounted, and imaged using Olympus laser scanning confocal microscope (Spin SR) or slide scanner of Zeiss (Axioscan 7)

Beads were washed with 20 mM and 40 mM imidazole steps and protein was eluted using 250 mM imidazole in 50 mM Tris-HCl, 100 mM NaCl, 0.5 mM MgCl 2 (pH 7.5)

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Therapeutic targeting of metabolic reprogramming in NSCLC Glycolysis inhibitors Targeting glycolysis in NSCLC has been explored as a therapeutic strategy, with compounds such as 2-deoxyglucose (2-DG) and PFKFB3 inhibitors showing promise in preclinical studies as presented in Fig